Journal: Molecular cell

Article Title: Differentiation drives widespread rewiring of the neural stem cell chaperone network

doi: 10.1016/j.molcel.2020.03.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-Tcp1δ (H-1) , Santa Cruz Biotech. , sc-137092: RRID: AB_2073768.

Techniques: Affinity Purification, Virus, Recombinant, Transfection, Cell Culture, Staining, Flow Cytometry, Fluorescence, Microscopy, Luminescence Assay, Imaging, Plasmid Preparation, Software

Journal: iScience

Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading

doi: 10.1016/j.isci.2025.112273

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-CCT2 , Santa Cruz , #sc-374152; RRID: AB_10917207.

Techniques: Recombinant, Selection, Staining, Negative Control, Software

Journal:

Article Title: Mutant forms of tumour necrosis factor receptor I that occur in TNF-receptor-associated periodic syndrome retain signalling functions but show abnormal behaviour

doi: 10.1111/j.1365-2567.2004.01942.x

Figure Lengend Snippet: Antibodies employed for confocal microscopy

Article Snippet: For staining with monodansylcadaverine (MDC) (Sigma), following indirect staining with antibody and washing, cells were incubated with 0·05 m m MDC in PBS at 37° for 10 min and then washed four times with PBS. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Species and isotype/preparation Source Secondary conjugate * Anti-TNFRSF1A Mouse mAb † IgG1 R & D Systems Europe Ltd, Abingdon, Oxon, UK Alexa Fluor 555 goat anti-mouse IgG1 Anti-HLA-ABC Mouse mAb IgG2a Serotec, Oxford, UK Alexa Fluor 488 goat anti-mouse IgG2a Anti-Hsc70 Mouse mAb IgM Affinity Bioreagents (through Cambridge Biosciences, Cambridge, UK) Alexa Fluor 647 goat anti-mouse IgM Anti-BiP/GRP78 Mouse mAb IgG2a BD Biosciences, Cowley, Oxford, UK Alexa Fluor 488 goat anti-mouse IgG2a Anti-HDJ2 Mouse mAb IgG1 Abcam, Cambridge, UK Alexa Fluor 488 goat anti-mouse IgG (H + L) Anti-TCP1 Rat mAb IgG2a Abcam, Cambridge, UK Alexa Fluor 647 goat anti-rat IgG (H + L) Anti-Ubiquitin Rabbit Ig fraction Dakocytomation Ltd, Ely, Cambs, UK Alexa Fluor 488 goat anti-rabbit IgG (H + L) Anti-SODD Rabbit polyclonal IgG Upstate Biotechnologies UK, Milton Keynes, UK Alexa Fluor 647 goat anti-rabbit IgG (H + L) Anti-TRADD Mouse mAb IgG2a Upstate Biotechnologies UK, Milton Keynes, UK Alexa Fluor 488 goat anti-mouse IgG2a Open in a separate window * All Alexa Fluor conjugates were purchased from Molecular Probes Europe, the Netherlands.

Techniques:

Journal:

Article Title: Mutant forms of tumour necrosis factor receptor I that occur in TNF-receptor-associated periodic syndrome retain signalling functions but show abnormal behaviour

doi: 10.1111/j.1365-2567.2004.01942.x

Figure Lengend Snippet: Surface and intracellular expression of recombinant full-length tumour necrosis factor receptor superfamily 1A (TNFRSF1A). Transfected HEK-293 cell lines (a), or clones (b), induced with doxycyclin to express the wild-type (WT) or different mutant full-length TNFRSF1A were stained with phycoerythrin-labelled anti-TNFRSF1A, without permeabilization, for surface staining, or with permeabilization for surface plus cytoplasmic staining, followed by flow cytometric analysis. Thin solid line, IgG1 isotype control; dashed line, surface staining with anti-TNFRSF1A; bold solid line, staining of permeabilized cells with anti-TNFRSF1A.

Article Snippet: For staining with monodansylcadaverine (MDC) (Sigma), following indirect staining with antibody and washing, cells were incubated with 0·05 m m MDC in PBS at 37° for 10 min and then washed four times with PBS. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Species and isotype/preparation Source Secondary conjugate * Anti-TNFRSF1A Mouse mAb † IgG1 R & D Systems Europe Ltd, Abingdon, Oxon, UK Alexa Fluor 555 goat anti-mouse IgG1 Anti-HLA-ABC Mouse mAb IgG2a Serotec, Oxford, UK Alexa Fluor 488 goat anti-mouse IgG2a Anti-Hsc70 Mouse mAb IgM Affinity Bioreagents (through Cambridge Biosciences, Cambridge, UK) Alexa Fluor 647 goat anti-mouse IgM Anti-BiP/GRP78 Mouse mAb IgG2a BD Biosciences, Cowley, Oxford, UK Alexa Fluor 488 goat anti-mouse IgG2a Anti-HDJ2 Mouse mAb IgG1 Abcam, Cambridge, UK Alexa Fluor 488 goat anti-mouse IgG (H + L) Anti-TCP1 Rat mAb IgG2a Abcam, Cambridge, UK Alexa Fluor 647 goat anti-rat IgG (H + L) Anti-Ubiquitin Rabbit Ig fraction Dakocytomation Ltd, Ely, Cambs, UK Alexa Fluor 488 goat anti-rabbit IgG (H + L) Anti-SODD Rabbit polyclonal IgG Upstate Biotechnologies UK, Milton Keynes, UK Alexa Fluor 647 goat anti-rabbit IgG (H + L) Anti-TRADD Mouse mAb IgG2a Upstate Biotechnologies UK, Milton Keynes, UK Alexa Fluor 488 goat anti-mouse IgG2a Open in a separate window * All Alexa Fluor conjugates were purchased from Molecular Probes Europe, the Netherlands.

Techniques: Expressing, Recombinant, Transfection, Clone Assay, Mutagenesis, Staining

Journal:

Article Title: Mutant forms of tumour necrosis factor receptor I that occur in TNF-receptor-associated periodic syndrome retain signalling functions but show abnormal behaviour

doi: 10.1111/j.1365-2567.2004.01942.x

Figure Lengend Snippet: Surface and intracellular expression of recombinant Δsig-tumour necrosis factor receptor superfamily 1A (TNFRSF1A). Transfected HEK-293 cell lines induced with doxycyclin to express the wild-type (WT) or different mutant Δsig-TNFRSF1A were stained with phycoerythrin-labelled anti-TNFRSF1A without permeabilization for surface staining, or with permeabilization for surface plus cytoplasmic staining, followed by flow cytometric analysis. Thin unbroken line, IgG1 isotype control; dashed line, surface staining with anti-TNFRSF1A; bold unbroken line, staining of permeabilized cells with anti-TNFRSF1A.

Article Snippet: For staining with monodansylcadaverine (MDC) (Sigma), following indirect staining with antibody and washing, cells were incubated with 0·05 m m MDC in PBS at 37° for 10 min and then washed four times with PBS. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Species and isotype/preparation Source Secondary conjugate * Anti-TNFRSF1A Mouse mAb † IgG1 R & D Systems Europe Ltd, Abingdon, Oxon, UK Alexa Fluor 555 goat anti-mouse IgG1 Anti-HLA-ABC Mouse mAb IgG2a Serotec, Oxford, UK Alexa Fluor 488 goat anti-mouse IgG2a Anti-Hsc70 Mouse mAb IgM Affinity Bioreagents (through Cambridge Biosciences, Cambridge, UK) Alexa Fluor 647 goat anti-mouse IgM Anti-BiP/GRP78 Mouse mAb IgG2a BD Biosciences, Cowley, Oxford, UK Alexa Fluor 488 goat anti-mouse IgG2a Anti-HDJ2 Mouse mAb IgG1 Abcam, Cambridge, UK Alexa Fluor 488 goat anti-mouse IgG (H + L) Anti-TCP1 Rat mAb IgG2a Abcam, Cambridge, UK Alexa Fluor 647 goat anti-rat IgG (H + L) Anti-Ubiquitin Rabbit Ig fraction Dakocytomation Ltd, Ely, Cambs, UK Alexa Fluor 488 goat anti-rabbit IgG (H + L) Anti-SODD Rabbit polyclonal IgG Upstate Biotechnologies UK, Milton Keynes, UK Alexa Fluor 647 goat anti-rabbit IgG (H + L) Anti-TRADD Mouse mAb IgG2a Upstate Biotechnologies UK, Milton Keynes, UK Alexa Fluor 488 goat anti-mouse IgG2a Open in a separate window * All Alexa Fluor conjugates were purchased from Molecular Probes Europe, the Netherlands.

Techniques: Expressing, Recombinant, Transfection, Mutagenesis, Staining